Multiple - Locus Variable - Number Tandem - Repeat Analysis of 1 Acinetobacter baumannii in China : comparison with PFGE typing
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چکیده
Acinetobacter baumannii in China: comparison with PFGE typing. 2 Yuan Hu, Boqing Li, Dazhi Jin, Zhigang Cui, Xiaoxia Tao, Binghua Zhang, 3 Jianzhong Zhang 4 5 1 State Key Laboratory for Infectious Disease Prevention and Control, National 6 Institute for Communicable Disease Control and Prevention, Chinese Center for 7 Disease Control and Prevention, 2 Binzhou Medical College, Yantai, china, 3 8 Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China. 9 10 *Correspondence auther: Professor Jian-Zhong Zhang, State Key Laboratory for 11 Infectious Disease Prevention and Control, National Institute for Communicable 12 Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 13 155 Changbai Road Changping District, Beijing, China 102206. Phone: 14 8610-58900707. E-mail: [email protected] 15 16 Abstract 17
منابع مشابه
Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis typing of Acinetobacter baumannii in China.
A panel of seven variable-number tandem-repeat (VNTR) markers was selected for Acinetobacter baumannii typing analysis (MLVA-7). Compared with pulsed-field gel electrophoresis (PFGE), MLVA-7 provided greater discrimination. We modified the criteria for MLVA complex assignments proposed previously, and a remarkable congruence between MLVA-7- and PFGE-based strain clustering was observed.
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In search of an effective DNA typing technique for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target sequence was evaluated. Using known genomic sequences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment le...
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Outbreak strains of Acinetobacter baumannii are highly clonal, and cross-infection investigations can be difficult. We sought targets based on AbaR resistance islands and on other genes found in some, but not all, sequenced isolates of A. baumannii among a set of clinical isolates (n = 70) that included multiple representatives of a number of pulsed-field gel electrophoresis (PFGE)-defined type...
متن کاملMultiple-locus variable-number tandem repeat analysis for typing of Staphylococcus epidermidis.
We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable...
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تاریخ انتشار 2013